Accessibility of Enzymatically Delignified Bambusa bambos for Efficient Hydrolysis at Minimum Cellulase Loading: An Optimization Study

Table 2

Experimental design (conditions and responses) for enzymatic saccharification of pretreated Bambusa bambos in terms of coded factor.

Run order	𝐵1	𝐵2	𝐵3	𝐵4	Reducing sugar (mg/g of substrate)
					Experimental	Predicted
(1)	−1 (4)	−1 (40)	−1 (16)	+1 (8)	671.21	671.15
(2)	+1 (8)	−1 (40)	−1 (16)	−1 (6)	691.45	698.26
(3)	−1 (4)	+1 (60)	−1 (16)	−1 (4)	621.67	632.60
(4)	+1 (8)	+1 (60)	–1 (16)	+1 (8)	639.10	656.21
(5)	−1 (4)	−1 (40)	+1 (20)	−1 (4)	695.47	683.00
(6)	+1 (8)	−1 (40)	+1 (20)	+1 (8)	697.92	691.63
(7)	−1 (4)	+1 (60)	+1 (20)	+1 (8)	700.13	705.95
(8)	+1 (8)	+1 (60)	+1 (20)	−1 (4)	701.25	705.95
(9)	+1 (8)	–1 (40)	−1 (16)	+1 (8)	698.32	693.32
(10)	−1 (4)	+1 (60)	−1 (16)	+1 (8)	702.23	692.28
(11)	+1 (8)	+1 (60)	−1 (16)	−1 (4)	657.13	647.90
(12)	−1 (4)	−1 (40)	+1 (20)	+1 (8)	667.27	675.42
(13)	+1 (8)	−1 (40)	+1 (20)	−1 (4)	741.71	750.58
(14)	−1 (4)	+1 (60)	+1 (20)	−1 (4)	696.09	696.60
(15)	+1 (8)	+1 (60)	+1 (20)	+1 (8)	660.43	659.35
(16)	−1 (4)	0 (50)	0 (18)	0 (6)	734.01	735.68
(17)	+1 (8)	0 (50)	0 (18)	0 (6)	767.35	751.44
(18)	0 (6)	−1 (40)	0 (18)	0 (6)	798.66	798.65
(19)	0 (6)	+1 (60)	0 (18)	0 (6)	801.12	786.90
(20)	0 (6)	0 (50)	−1 (16)	0 (6)	758.24	747.63
(21)	0 (6)	0 (50)	+1 (20)	0 (6)	782.41	778.79
(22)	0 (6)	0 (50)	0 (18)	0 (6)	801.23	808.36
(23)	0(6)	0(50)	0 (18)	0(6)	797.39	808.36
(24)	0 (6)	0 (50)	0 (18)	−1 (4)	808.56	805.25
(25)	0 (6)	0 (50)	0 (18)	+1 (8)	809.72	805.61
(26)	0 (6)	0 (50)	0 (18)	0 (6)	801.43	808.36
(27)	0 (6)	0 (50)	0 (18)	0 (6)	795.12	808.36
(28)	0 (6)	0 (50)	0 (18)	0 (6)	811.06	808.36
(29)	0 (6)	0 (50)	0 (18)	0 (6)	810.06	808.36
(30)	0 (6)	0 (50)	0 (18)	0 (6)	802.62	808.36
(31)	0 (6)	0 (50)	0 (18)	0 (6)	817.31	808.36
(32)	0 (6)	0 (50)	0 (18)	0 (6)	803.08	808.36