Cellulolytic Enzymes Production via Solid-State Fermentation: Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate

Table 1

Composition of various substrates (dry basis).

Sample	Cellulose (ADF-ADL)	Hemicellulose (NDF-ADF)	Holocellulose*	Lignin (ADL)	Protein	Ash
Native soybean hulls	45.90±0.60	19.59 ± 0.57	65.48 ± 1.14^A	0.75 ± 0.09	11.96 ± 0.06	5.21 ± 0.01
Steam-treated soybean hulls	49.99 ± 2.67	19.32 ± 0.83	69.31 ± 3.38^A	1.19 ± 0.15	10.43 ± 0.07	2.67 ± 0.05
HCl-treated soybean hulls	57.19 ± 0.40	15.33 ± 0.96	72.52 ± 1.08^A	1.33 ± 0.07	9.60 ± 0.03	2.55 ± 0.05
H2SO4-treated soybean hulls	54.74 ± 0.47	17.39 ± 0.77	72.14 ± 1.23^A	1.47 ± 0.19	10.11 ± 0.11	2.78 ± 0.08
NaOH-treated soybean hulls	60.45 ± 1.61	15.66 ± 1.58	76.11 ± 3.15^A	1.23 ± 0.03	3.45 ± 0.06	3.26 ± 0.06

*Represents sum of cellulose and hemicellulose; data are expressed as mean ± SE; 𝑛=4; means with same letters do not differ significantly. Pairwise comparisons between total cellulosics were tested using Tukey Kramer HSD at 𝑃<.05.