Cellulolytic Enzymes Production via Solid-State Fermentation: Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate

Table 3

Effect of interaction between crystallinity and bed porosity of substrates on cellulolytic enzyme production in both mono and mixed SSF of T. reesei and A. oryzae.

Interaction	Culture	Cellulolytic enzyme system				Treatments considered
		Filter paper units (FPU/g-ds)	β-glucosidase (IU/g-ds)	Endoglucanase (IU/g-ds)	Xylanase (IU/g-ds)
Crystallinity × porosity	Trichoderma reesei	<0.0001*	0.0388*	<0.0001*	0.0472	Native, steam
Crystallinity × porosity	Aspergillus oryzae	0.4629	0.9218	0.0005*	0.9912	Native, steam
Crystallinity × porosity	Mixed	0.0044*	0.0449	0.0257**	0.9061	Native, steam

*Indicates Tukey probability for a particular interaction is significant at 95% confidence. **Indicates significance at 𝑃<.05 but not significance at 𝑃<.01. Model (9) ran in SAS 9.1.
Abbreviations: native, untreated soybean hulls; steam, steam-pretreated soybean hulls.