Production, Purification, and Characterization of Polygalacturonase from Rhizomucor pusillus Isolated from Decomposting Orange Peels

Table 2

Purification of polygalacturonase from Rhizomucor pusilis.

Purification step	Total activity (U/mL)	Total protein (mg)	Specific activity (U/mg)	Fold purification	Yield (%)
Crude preparation	31.74	6.38	4.97	1	100
Sephadex G-200	13.23	0.42	31.50	6.33	41.68
Sephacryl S-100	8.59	0.14	61.35	12.34	27.06