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Enzyme Research
Volume 2012 (2012), Article ID 157643, 7 pages
http://dx.doi.org/10.1155/2012/157643
Research Article

Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

Department of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamilnadu, India

Received 27 August 2012; Accepted 30 October 2012

Academic Editor: Ali-Akbar Saboury

Copyright © 2012 P. Saravanan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.