Research Article
The Role of Arg13 in Protein Phosphatase M tPphA from Thermosynechococcus elongatus
Table 2
Kinetic parameters of tPphA and Arg-13 variants towards pNPP. pNPP assays were carried out as described in Experimental Procedures (Subsection 2.2). The pH of the reaction was 8.3. From the apparent reaction velocities of three independent repetitions, the kinetic parameters were calculated by linear fitting using the program GraphPad Prism 4. ± indicates standard error. The results were obtained from single preparations of the wild-type (WT) and variant tPphA purifications.
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—: not detectable. |