Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 2

Coded levels and real values (in parentheses) for the second (12 trials) and third (13 trials) factorial design for free and immobilized lipase activity.

Run	pH**	T (°C)	FLCS* (U/mL supernatant)	IE* (U/g-support)
1	−1 (4)	−1 (36)	28	9
2	+1 (8)	−1 (36)	20	11
3	−1 (4)	+1 (64)	128	12
4	+1 (8)	+1 (64)	13	13
5	−1.41 (3)	0 (50)	79	12
6	+1.41(9)	0 (50)	0	35
7	0 (6)	−1.41 (30)	37	9
8	0 (6)	+1.41 (70)	145	51
9	0 (6)	0 (50)	119	74
10	0 (6)	0 (50)	122	62
11	0 (6)	0 (50)	119	74
12	0 (6)	0 (50)	123	69
13	0 (6)	0 (50)	—	69

*FLCS: free enzyme and IE: immobilized enzyme.
**Buffer solutions: 0.05 M citrate-phosphate buffer for pH 3.0, 4.0, 5.0, 6.0, and 7.0; 0.05 M Tris-HCl buffer for pH 8.0 and 9.0.