Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 3

Temperature stability of the free and immobilized lipase.*

Temperature	Relative activity (%)***
	Free lipase (FLCS)	Immobilized lipase (IE)
Control**	100^d,e,f	100^d,e,f
30°C	50±8.24^k	102±15.1^d,e
40°C	51±2.35^j,k	91±9.0^e,f,g
50°C	85±0^e,f,g,h,i	227±0^a
60°C	87±5.23^e,f,g,h	143±0^b,c
70°C	53±0^j,k	123±3.7^c,d

*The free and immobilized enzymes were incubated at different temperatures for 2 h.
**Control: free and immobilized lipase without incubation.
***Mean values with the same letter do not statistically differ from each other by the ANOVA Tukey test (𝑃=0.01).