Enzyme Research

Enzyme Research / 2012 / Article / Tab 6

Research Article

Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 6

Stability of P. hubeiensis free and immobilized lipase activity in organic solvents.*

Organic solventConcentration (%)Relative activity (%)***
Free lipase (FLCS)Immobilized lipase (IE)

Control**100b,c,d100b,c,d

Acetone20 b,c,d,e b,c,d
50 i,j a,b,c,d
80 j,k b,c,d

Methanol20 b,c,d,e a
50 h,i,j,k b
80 k,l d,e,f,g

Ethanol20 a,b c,d,e,f
50 h,i,j i,j
800k j,k

2-propanol20 c,d,e,f c,d,e,f
50 k,j a,b,c
80 i,j,k a,b,c,d

Butanol20 a,b,c,d g,h,i
50 d,e,f,g,h b,c,d,e
80 b,c,d,e,f c,d,e,f,g

*The free and immobilized enzymes were incubated in the presence of various organic solvents at 50°C for 1h.
**Control: free and immobilized lipase without the addition of any substance.
***Mean values with the same letter do not statistically differ from each other by the ANOVA Tukey test ( ).

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