Inhibition of Heme Peroxidases by Melamine

Table 1

Enzyme kinetic parameters for horseradish peroxidase and lactoperoxidase.

	HRP^a	LPO^b
𝑉max	14.63±0.15𝜇M/min	2.60±0.10mM/sec
𝐾𝑚	1.67±0.06mM	2.9±0.4mM
𝐾𝑖	9.5±0.7mM	15±5mM
Type of inhibition	Noncompetitive	Mixed model (primarily competitive)

^a𝑉_max and 𝐾_𝑚 for HRP refer to ABTS as the substrate. ^b𝑉_max and 𝐾_𝑚 for LPO refer to potassium iodide as the substrate.