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Enzyme Research
Volume 2012, Article ID 542589, 5 pages
http://dx.doi.org/10.1155/2012/542589
Research Article

Hydrolysis of Virgin Coconut Oil Using Immobilized Lipase in a Batch Reactor

1Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor, 81310 Johor Bahru, Malaysia
2Department of Chemical Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, Johor, 81310 Johor Bahru, Malaysia
3Deparment of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, Johor, 81310 Johor Bahru, Malaysia

Received 5 April 2012; Accepted 22 July 2012

Academic Editor: Ali-Akbar Saboury

Copyright © 2012 Lee Suan Chua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hydrolysis of virgin coconut oil (VCO) had been carried out by using an immobilised lipase from Mucor miehei (Lipozyme) in a water-jacketed batch reactor. The kinetic of the hydrolysis was investigated by varying the parameters such as VCO concentration, enzyme loading, water content, and reaction temperature. It was found that VCO exhibited substrate inhibition at the concentration more than 40% (v/v). Lipozyme also achieved the highest production of free fatty acids, 4.56 mM at 1% (w/v) of enzyme loading. The optimum water content for VCO hydrolysis was 7% (v/v). A relatively high content of water was required because water was one of the reactants in the hydrolysis. The progress curve and the temperature profile of the enzymatic hydrolysis also showed that Lipozyme could be used for free fatty acid production at the temperature up to 50°C. However, the highest initial reaction rate and the highest yield of free fatty acid production were at 45 and 40°C, respectively. A 100 hours of initial reaction time has to be compensated in order to obtain the highest yield of free fatty acid production at 40°C.