Table 2: Reaction specificities of pullulan-degrading enzymes.

Enzyme EC number Bonds processed Preferred substrate End products References

Pullulanase type I
3.2.1.41α-(1,6) Oligo- and polysaccharides, Pullulan Trimer (maltotriose) [17, 27]

Pullulanase type II
(amylopullulanase)
α-(1,6)Pullulan, Trimer (maltotriose)
3.2.1.41α-(1,4)Poly- and oligosaccharide (starch)Mixture of glucose, maltose, and maltotriose [31, 32, 40]

Pullulan hydrolase type I
(neopullulanase)
3.2.1.135α-(1,4)PullulanPanose[4143]

Pullulan hydrolase type II
(isopullulanase)
3.2.1.57α-(1,4)PullulanIsopanose[12]

Pullulan hydrolase type IIIα-(1,4) and
α-(1,6)
PullulanMixture of panose, maltose, and maltotriose
3.2.1.—Starch, amylose, and amylopectinMaltotriose and maltose [37]