Table 2: Reaction specificities of pullulan-degrading enzymes.

Enzyme EC number Bonds processed Preferred substrate End products References

Pullulanase type Iα-(1,6) Oligo- and polysaccharides, Pullulan Trimer (maltotriose) [17, 27]

Pullulanase type II
α-(1,6)Pullulan, Trimer (maltotriose)α-(1,4)Poly- and oligosaccharide (starch)Mixture of glucose, maltose, and maltotriose [31, 32, 40]

Pullulan hydrolase type I

Pullulan hydrolase type II

Pullulan hydrolase type IIIα-(1,4) and
PullulanMixture of panose, maltose, and maltotriose
3.2.1.—Starch, amylose, and amylopectinMaltotriose and maltose [37]