Enzyme Research

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Review Article

Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications

Table 5

Physicochemical properties of selected pullulan-hydrolyzing enzymes from various microorganisms.

Organism 𝑀 π‘Ÿ a (kDa)
pIOptimal  pHOptimal temperature (Β°C)Reference

Type I pullulanase     
 Bacillus flavocaldarius KP 122855n.d.b7.075–80[51]
 Streptomyces sp. No. 27n.d.n.d.4–755–60[52]
 Thermus caldophilus GK-24656.15.575[27]
 Fervidobacterium pennavorans Ven 5240n.d.6.085[28]
 Bacillus thermoleovorans US 105 (expressed in E. coli) n.d.n.d.5.6–6.075[30]
Type II pullulanase     
 Thermoanaerobacter strain B6A4504.55.075[53]
 Clostridium thermosulfurigenes EM 1102n.d.5.5–6.060–65[54]
 Pyrococcus furiosus 110n.d.5.598[55]
 Thermococcus litoralis 119n.d.5.598[55]
 Desulfurococcus mucosus n.d.n.d.5.0100[56]
 Thermococcus celer n.d.n.d.5.590[56]
 Thermococcus strain TYn.d.n.d.6.5100[56]
 Pyrococcus woesei 90 n.d. 6.0 100 [26]
 Thermococcus hydrothermalis 110–128n.d.5.595[14]
 Desulfurococcus mucosus expressed in Bacillus subtilis 66n.d.5.085[32]
 Bacillus stearothermophilus expressed in B. subtilis 62
 Bacillus stearothermophilus TRS 4062n.d.6.060–65 [42]
 Bacteroides thetaiotaomicron 95-170n.d.6.537[57]
 Bacillus polymyxa CECT 155n.d.n.d.6.050[58]
 Aspergillus niger ATCC 964269–71 n.d.3.540[36]
 Bacillus sp. US 149 200n.d.5.060[31]
Alkaline amylopullulanase     
 Bacillus sp. KSM-18761205.210–10.5 50[59]
 Bacillus sp. KSM 13782104.89.550[41]
Pullulan hydrolase type III     
 Thermococcus aggregans expressed in E. coli n.d.

aMolecular mass; bnot detected by the researchers.

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