|
| Organism | (kDa)
| pI | Optimal pH | Optimal temperature (°C) | Reference |
|
| Type I pullulanase | | | | | |
| Bacillus flavocaldarius KP 1228 | 55 | n.d.b | 7.0 | 75–80 | [51] |
| Streptomyces sp. No. 27 | n.d. | n.d. | 4–7 | 55–60 | [52] |
| Thermus caldophilus GK-24 | 65 | 6.1 | 5.5 | 75 | [27] |
| Fervidobacterium pennavorans Ven 5 | 240 | n.d. | 6.0 | 85 | [28] |
| Bacillus thermoleovorans US 105 (expressed in E. coli) | n.d. | n.d. | 5.6–6.0 | 75 | [30]
|
| Type II pullulanase | | | | | |
| Thermoanaerobacter strain B6A | 450 | 4.5 | 5.0 | 75 | [53] |
| Clostridium thermosulfurigenes EM 1 | 102 | n.d. | 5.5–6.0 | 60–65 | [54] |
| Pyrococcus furiosus | 110 | n.d. | 5.5 | 98 | [55] |
| Thermococcus litoralis | 119 | n.d. | 5.5 | 98 | [55] |
| Desulfurococcus mucosus | n.d. | n.d. | 5.0 | 100 | [56] |
| Thermococcus celer | n.d. | n.d. | 5.5 | 90 | [56] |
| Thermococcus strain TY | n.d. | n.d. | 6.5 | 100 | [56] |
| Pyrococcus woesei | 90 | n.d. | 6.0 | 100 | [26] |
| Thermococcus hydrothermalis | 110–128 | n.d. | 5.5 | 95 | [14] |
| Desulfurococcus mucosus expressed in Bacillus subtilis | 66 | n.d. | 5.0 | 85 | [32] |
| Neopullulanases | | | | | |
| Bacillus stearothermophilus expressed in B. subtilis | 62
| n.d.
| 6.0
| 60–65
| [42] |
| Bacillus stearothermophilus TRS 40 | 62 | n.d. | 6.0 | 60–65 | [42] |
| Bacteroides thetaiotaomicron 95-1 | 70 | n.d. | 6.5 | 37 | [57] |
| Bacillus polymyxa CECT 155 | n.d. | n.d. | 6.0 | 50 | [58]
|
| Isopullulanases | | | | | |
| Aspergillus niger ATCC 9642 | 69–71 | n.d. | 3.5 | 40 | [36] |
| Bacillus sp. US 149 | 200 | n.d. | 5.0 | 60 | [31] |
| Alkaline amylopullulanase | | | | | |
| Bacillus sp. KSM-1876 | 120 | 5.2 | 10–10.5 | 50 | [59] |
| Bacillus sp. KSM 1378 | 210 | 4.8 | 9.5 | 50 | [41]
|
| Pullulan hydrolase type III | | | | | |
| Thermococcus aggregans expressed in E. coli | n.d.
| n.d.
| 6.5 | 95 | [37] |
|