Research Article
Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra
Table 1
Summary of purification of thermostable lipase from G. thermodentrificans.
| Purification step fold
| Total activity (U) | Total Protein (mg) | Specific activity (U/mg) | Yield (%) | Fold |
| | Crude lipase | 4167.0 | 3840.0 | 1.09 | 100.0 | 1 | | Ultrafiltration | 3088.82 | 942.28 | 3.28 | 74.1 | 3.0 | | Affinity Chromatography | 1232.0 | 144.32 | 8.54 | 29.6 | 7.8 | | Gel-filtration | 368.88 | 10.05 | 36.7 | 8.9 | 33.7 |
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