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Enzyme Research
Volume 2013 (2013), Article ID 287343, 10 pages
http://dx.doi.org/10.1155/2013/287343
Research Article

An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues

1Department of Cellular Biology, Institute of Biological Sciences, University of Brasilia, 70.910-900 Brasilia, DF, Brazil
2Department of Biochemistry and Molecular Biology, Institute of Biological Sciences, Federal University of Goias, 74.001-970 Goiania, GO, Brazil
3Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, ET 18/25, Darcy Ribeiro University Campus, 70.910-900 Brasilia, DF, Brazil

Received 19 March 2013; Revised 11 June 2013; Accepted 13 June 2013

Academic Editor: Joaquim Cabral

Copyright © 2013 Eveline Queiroz de Pinho Tavares et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A) was efficiently secreted as a 35 kDa protein which was purified through a two-step chromatography procedure. The highest enzyme activity was detected at C/pH 4. rEG A retained 100% of activity when incubated at 45 and C for 72 h. Purified rEG A kinetic parameters towards CMC were determined as  mg/mL,  mmol/min, and 55.8 IU (international units)/mg specific activity. Recombinant P. pastoris supernatant presented hydrolytic activity towards lignocellulosic residues such as banana stalk, sugarcane bagasse, soybean residues, and corn straw. These data indicate that rEG A is suitable for plant biomass conversion into products of commercial importance, such as second-generation fuel ethanol.