Table of Contents Author Guidelines Submit a Manuscript
Enzyme Research
Volume 2013 (2013), Article ID 597028, 4 pages
http://dx.doi.org/10.1155/2013/597028
Research Article

Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii

Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India

Received 31 December 2012; Accepted 28 March 2013

Academic Editor: Munishwar Nath Gupta

Copyright © 2013 Juhi Sikarwar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Howard, M. O’Donoghue, A. Feeney, and R. D. Sleator, “Acinetobacter baumannii: an emerging opportunistic pathogen,” Virulence, vol. 3, no. 3, pp. 1–8, 2012. View at Publisher · View at Google Scholar
  2. B. A. Eijkelkamp, K. A. Hassan, I. T. Paulsen, and M. H. Brown, “Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions,” BMC Genomics, vol. 12, article 126, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. T. D. Gootz and A. Marra, “Acinetobacter baumannii: an emerging multidrug-resistant threat,” Expert Review of Anti-Infective Therapy, vol. 6, no. 3, pp. 309–325, 2008. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Bassetti, E. Righi, S. Esposito, N. Petrosillo, and L. Nicolini, “Drug treatment for multidrug-resistant Acinetobacter baumannii infections,” Future Microbiology, vol. 3, no. 6, pp. 649–660, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. N. C. Gordon and D. W. Wareham, “Multidrug-resistant Acinetobacter baumannii: mechanisms of virulence and resistance,” International Journal of Antimicrobial Agents, vol. 35, no. 3, pp. 219–226, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. I. Lascu, “The nucleoside diphosphate kinases 1973–2000,” Journal of Bioenergetics and Biomembranes, vol. 32, no. 3, pp. 213–214, 2000. View at Google Scholar · View at Scopus
  7. A. M. Chakrabarty, “Nucleoside diphosphate kinase: role in bacterial growth, virulence, cell signalling and polysaccharide synthesis,” Molecular Microbiology, vol. 28, no. 5, pp. 875–882, 1998. View at Publisher · View at Google Scholar · View at Scopus
  8. G. W. Sundin, S. Shankar, and A. M. Chakrabarty, “Mutational analysis of nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of critical amino acid residues involved in exopolysaccharide alginate synthesis,” Journal of Bacteriology, vol. 178, no. 24, pp. 7120–7128, 1996. View at Google Scholar · View at Scopus
  9. Z. Jiang, A. I. Vasil, L. Gera, M. L. Vasil, and R. S. Hodges, “Rational design of α-helical antimicrobial peptides to target Gram-negative pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa: utilization of charge, ‘specificity determinants,’ total hydrophobicity, hydrophobe type and location as design parameters to improve the therapeutic ratio,” Chemical Biology and Drug Design, vol. 77, no. 4, pp. 225–240, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. N. Kimura, “Introduction: nucleoside diphosphate kinases: genes and protein functions,” Journal of Bioenergetics and Biomembranes, vol. 35, no. 1, pp. 3–4, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Sun, X. Wang, A. Lau, T. Y. A. Liao, C. Bucci, and Z. Hmama, “Mycobacterial nucleoside diphosphate kinase blocks phagosome maturation in murine raw 264.7 macrophages,” PLoS One, vol. 5, no. 1, Article ID e8769, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. P. S. Steeg, M. Zollo, and T. Wieland, “A critical evaluation of biochemical activities reported for the nucleoside diphosphate kinase/Nm23/Awd family proteins: opportunities and missteps in understanding their biological functions,” Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 384, no. 4-5, pp. 331–339, 2008. View at Google Scholar
  13. R. Spooner and Ö. Yilmaz, “Nucleoside-diphosphate-kinase: a pleiotropic effector in microbial colonization under interdisciplinary characterization,” Microbes and Infection, vol. 14, no. 3, pp. 228–237, 2012. View at Publisher · View at Google Scholar
  14. S. Arai, Y. Yonezawa, N. Okazaki et al., “A structural mechanism for dimeric to tetrameric oligomer conversion in Halomonas sp. nucleoside diphosphate,” Protein Science, vol. 21, no. 4, pp. 498–510, 2012. View at Publisher · View at Google Scholar
  15. J. Janin, C. Dumas, S. Morera et al., “Three-dimensional structure of nucleoside diphosphate kinase,” Journal of Bioenergetics and Biomemberane, vol. 32, no. 3, pp. 215–255, 2000. View at Publisher · View at Google Scholar
  16. C. J. Chen, M. Y. Liu, T. Chang, W. C. Chang, B. C. Wang, and J. Le Gall, “Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans: coexpression of its activity with a Mn-superoxide dismutase,” Journal of Structural Biology, vol. 142, no. 2, pp. 247–255, 2003. View at Publisher · View at Google Scholar
  17. I. Lascu, A. Chaffotte, B. Limbourg-Bouchon, and M. Veron, “A pro/ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme,” Journal of Biological Chemistry, vol. 267, no. 18, pp. 12775–12781, 1992. View at Google Scholar · View at Scopus
  18. D. Deville-Bonne, O. Sellam, F. Merola, I. Lascu, M. Desmadril, and M. Véron, “Phosphorylation of nucleoside diphosphate kinase at the active site studied by steady-state and time-resolved fluorescence,” Biochemistry, vol. 35, no. 46, pp. 14643–14650, 1996. View at Publisher · View at Google Scholar · View at Scopus
  19. B. Schneider, A. Norda, A. Karlsson, M. Veron, and D. Deville-Bonne, “Nucleotide affinity for a stable phosphorylated intermediate of nucleoside diphosphate kinase,” Protein Science, vol. 11, no. 7, pp. 1648–1656, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. M. B. Charland, Sigma Plot or Scientists, Version 8.0, Wm. C. Brown Communications, Inc., Dubuque, Iowa, USA, 1995.