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Enzyme Research
Volume 2013, Article ID 784973, 8 pages
Research Article

Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans

Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6

Received 24 September 2012; Revised 17 December 2012; Accepted 17 January 2013

Academic Editor: Paul Engel

Copyright © 2013 Neal J. Dawson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material contains a table that outlines the purification scheme and typical yield for white muscle LDH from the anoxia-tolerant T. s. elegans. The results closely resemble those shown for control T. s. elegans within the article (Table 1). Additionally, the supplementary material contains a representative kinetic plot showing the activity of control and anoxic LDH with respect to changing lactate concentrations. This figure further demonstrates the dramatic increase in anoxic LDH Vmax as well as the decrease in anoxic LDH Km lactate.

  1. Supplementary Material