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Enzyme Research
Volume 2013, Article ID 897420, 9 pages
http://dx.doi.org/10.1155/2013/897420
Research Article

Recovery of Extracellular Lipolytic Enzymes from Macrophomina phaseolina by Foam Fractionation with Air

Laboratório de Tecnologia Bioquímica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenidal Ipiranga 2752, Sala 709, 90610-000 Porto Alegre, RS, Brazil

Received 26 February 2013; Revised 7 April 2013; Accepted 8 April 2013

Academic Editor: Denise M. Guimarães Freire

Copyright © 2013 Claudia Schinke and José Carlos Germani. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Macrophomina phaseolina was cultivated in complex and simple media for the production of extracellular lipolytic enzymes. Culture supernatants were batch foam fractionated for the recovery of these enzymes, and column design and operation included the use of P 2 frit (porosity 40 to 100 μm), air as sparging gas at variable flow rates, and Triton X-100 added at the beginning or gradually in aliquots. Samples taken at intervals showed the progress of the kinetic and the efficiency parameters. Best results were obtained with the simple medium supernatant by combining the stepwise addition of small amounts of the surfactant with the variation of the air flow rates along the separation. Inert proteins were foamed out first, and the subsequent foamate was enriched in the enzymes, showing estimated activity recovery (R), enrichment ratio (E), and purification factor (P) of 45%, 34.7, and 2.9, respectively. Lipases were present in the enriched foamate.