Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12
Figure 5
Multiple sequence alignment between amino acid sequence of PI12 protease with other subtilisin. The serine protease sequences were obtained from the GenBank database. Their accession numbers are: EMS20793 (subtilisin-like protease of Rhodosporidium toruloides NP11), CAG25549 (subtilisin-like protease of Pleurotus ostreatus), EPQ55564 (subtilisin-like protease of Gloeophyllum trabeum ATCC 11539), and ETW80907 (serine protease S8 of Heterobasidion irregulare TC 32-1). Putative ORF of serine protease from G. antarctica PI12 was labeled as PI12 protease.
