Research Article

Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Figure 7

Effect of postinduction time towards protease activity. The highest activity was detected at 72 hours after 110 hours of incubation, with 9 U/mL activity followed by 48, 24 and 96 hours. The substrate (azocasein) was dissolved in Tris HCL buffer and incubated for 30 min at 20°C. The absorbance was measured at 450 nm after 15 min incubation. A one-way ANOVA was used to test for preference differences among six different postinduction times. The statistical value ( value <0.05) between hours is 0.003.
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