Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Table 1

List of web servers applied for full-length cDNA of PI12 protease analysis.

Analysis	Web server/Software	Website
(1) Open reading frame (ORF); (2) Amino acid translation; (3) Intron-exon boundaries	Augustus Web server	http://augustus.gobics.de/
(1) Amino acid composition; (2) Molecular mass; (3) Isoelectric point	ProtParam	http://www.expasy.org/tool
Homology search	(1) NCBI BLASTX	http://www.ncbi.nlm.nih.gov
	(2) NCBI BLASTP
Phylogenetic tree	(1) Molecular Evolutionary Genetics Analysis (MEGA 6)	http://www.megasoftware.net/
	(2) CLUSTALW	http://seqtool.sdsc.edu/CGI/BW.cgi
Propeptide	ProP	http://www.cbs.dtu.dk/services/ProP-1.0/
Open reading frame	ORF Finder	http://www.ncbi.nlm.nih.gov/projects/gorf
Signal peptide	SignalP	http://www.cbs.dtu.dk/services/SignalP
Hydrophobic profile	ProtScale	http://au.expasy.org/cgi-bin
Multiple sequence alignment	Biology Workbench	http://workbench.sdsc.edu/
Protein motifs	ScanProsite	http://prosite.expasy.org/scanprosite/
N-glycosylation	NetNGlyc	http://www.cbs.dtu.dk/services/NetNGlyc/