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Enzyme Research
Table of Contents
Special Issues
Enzyme Research
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2014
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Article
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Tab 1
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Research Article
Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast
Glaciozyma antarctica
PI12
Table 1
List of web servers applied for full-length cDNA of PI12 protease analysis.
Analysis
Web server/Software
Website
(1) Open reading frame (ORF)
(2) Amino acid translation
(3) Intron-exon boundaries
Augustus Web server
http://augustus.gobics.de/
(1) Amino acid composition
(2) Molecular mass
(3) Isoelectric point
ProtParam
http://www.expasy.org/tool
Homology search
(1) NCBI BLASTX
http://www.ncbi.nlm.nih.gov
(2) NCBI BLASTP
Phylogenetic tree
(1) Molecular Evolutionary Genetics Analysis (MEGA 6)
http://www.megasoftware.net/
(2) CLUSTALW
http://seqtool.sdsc.edu/CGI/BW.cgi
Propeptide
ProP
http://www.cbs.dtu.dk/services/ProP-1.0/
Open reading frame
ORF Finder
http://www.ncbi.nlm.nih.gov/projects/gorf
Signal peptide
SignalP
http://www.cbs.dtu.dk/services/SignalP
Hydrophobic profile
ProtScale
http://au.expasy.org/cgi-bin
Multiple sequence alignment
Biology Workbench
http://workbench.sdsc.edu/
Protein motifs
ScanProsite
http://prosite.expasy.org/scanprosite/
N-glycosylation
NetNGlyc
http://www.cbs.dtu.dk/services/NetNGlyc/