Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

Table 3

Experimental results of the path of steepest ascent.

Variable	Soluble starch (g/L)	Yeast extract (g/L)	K2HPO4 (g/L)	MgSO4·7H2O (g/L)	Glucoamylase activity (U/mL)
Base point (zero level in the PBD)	10	3	1	1
Origin step unit (concentration range of unity level)	5	2	0.5	0.5
Slope (estimated coefficient ratio from equation)	0.6525	0.4425	0.3775	0.2792
New step unit (slope × origin step unit)	3.26	0.89	0.19	0.14
Run 1	13.26	3.89	1.19	1.14	5.16
Run 2	16.52	4.78	1.38	1.28	5.91
Run 3	19.78	5.67	1.57	1.42	6.88
Run 4	23.04	6.56	1.76	1.56	8.13
Run 5	26.3	7.45	1.95	1.7	9.42
Run 6	29.56	8.34	2.14	1.84	10.48
Run 7	32.82	9.23	2.33	1.98	11.64
Run 8	36.08	10.12	2.52	2.12	10.28
Run 9	39.34	11.01	2.71	2.26	9.67
Run 10	42.6	11.9	2.9	2.4	9.21