Enzyme Research

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Research Article

Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

Table 3

Experimental results of the path of steepest ascent.

VariableSoluble starch (g/L)Yeast extract (g/L)K2HPO4 (g/L) · (g/L)Glucoamylase activity (U/mL)

Base point (zero level in the PBD)10311
Origin step unit (concentration range of unity level)520.50.5
Slope (estimated coefficient ratio from equation)0.65250.44250.37750.2792
New step unit (slope × origin step unit)3.260.890.190.14
Run 113.263.891.191.145.16
Run 216.524.781.381.285.91
Run 319.785.671.571.426.88
Run 423.046.561.761.568.13
Run 526.37.451.951.79.42
Run 629.568.342.141.8410.48
Run 732.829.232.331.9811.64
Run 836.0810.122.522.1210.28
Run 939.3411.012.712.269.67
Run 1042.611.92.92.49.21

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.