Research Article

Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation

Table 4

Influence of substances on PLIII activity from A. giganteus.

Substance (2 mM)PL activity (%)*

CoCl2153.6 ± 6.6
Pb(CH3COO)2134.7 ± 3.2
BaCl2132.4 ± 10.4
MgSO4125.8 ± 12.6
ZnSO4116.9 ± 4.8
NaCl110.6 ± 5.6
CaCl2107.4 ± 2.8
HgCl294.0 ± 8.9
MnSO488.5 ± 3.7
Sodium citrate96.6 ± 0.6
EDTA85.8 ± 7.4
DTT102.4 ± 3.0
-Mercaptoethanol78.8 ± 5.0
Iodoacetic acid75.1 ± 2.3
PMSF67.2 ± 2.5
SDS6.4 ± 0.9

Control: 100% ± 6.1.