Esterase Active in Polar Organic Solvents from the Yeast Pseudozyma sp. NII 08165

Figure 6

Purification of LIP1 esterase. (a) Zymogram analysis of Pseudozyma esterases. Lanes: 1: crude enzyme, 2: ammonium sulfate fraction. (b) Native PAGE analysis of esterases. Lanes: 1: crude enzyme, 2: ammonium sulfate fraction. (c) SDS PAGE analysis of purified LIP1, Lane 1: purified LIP1 esterase, Lane M: SDS broad range protein ladder (Spectra, Thermo Scientific). The 175 kD LIP1 esterase protein band is indicated by arrow head.

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