Esterase Active in Polar Organic Solvents from the Yeast Pseudozyma sp. NII 08165

Table 2

Box Behnken design matrix and responses obtained for the response surface optimization of esterase production by Pseudozyma sp.

Std. order	Inoculum conc. (% v/v)	pH	Incubation time (h)	Esterase activity (UmL^−1)
				Obtained	Predicted
1	2	4.0	84	419	63
2	4	4.0	84	764	213
3	2	5.0	84	2897	3447
4	4	5.0	84	7970	8325
5	2	4.5	72	1633	721
6	4	4.5	72	5251	4533
7	2	4.5	96	658	1375
8	4	4.5	96	1679	2591
9	3	4.0	72	474	1742
10	3	5.0	72	6267	6629
11	3	4.0	96	598	237
12	3	5.0	96	8113	6846
13	3	4.5	84	7601	6729
14	3	4.5	84	7806	6729
15	3	4.5	84	7652	6729
16	3	4.5	84	4472	6729
17	3	4.5	84	6113	6729