Research Article
Esterase Active in Polar Organic Solvents from the Yeast Pseudozyma sp. NII 08165
Table 3
Summary of purification of esterase from Pseudozyma sp.
| | Volume (mL) | Esterase activity (U) | Total activity (U) | Protein content (mg mL−1) | Total protein (mg) | Specific activity (Umg−1) | Fold purification |
| Crude enzyme | 1950 | 100 | 195390 | 2.2 | 4327 | 45 | 100 | Ultra filtration (50 kD) Retentate | 160 | 1203 | 192483 | 3.14 | 502 | 383 | 851 | 60% (NH4)2SO4 fraction | 17.5 | 3179 | 55638 | 2.7 | 47 | 1184 | 2631 | HIC fraction | 40 | 314 | 12560 | 0.085 | 3.4 | 3694 | 8208 |
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