Esterase Active in Polar Organic Solvents from the Yeast Pseudozyma sp. NII 08165

Table 3

Summary of purification of esterase from Pseudozyma sp.

	Volume; (mL)	Esterase activity; (U)	Total activity; (U)	Protein content; (mg mL^−1)	Total protein; (mg)	Specific activity; (Umg^−1)	Fold purification
Crude enzyme	1950	100	195390	2.2	4327	45	100
Ultra filtration (50 kD) Retentate	160	1203	192483	3.14	502	383	851
60% (NH4)2SO4 fraction	17.5	3179	55638	2.7	47	1184	2631
HIC fraction	40	314	12560	0.085	3.4	3694	8208