Research Article
Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69
Table 2
Plackett-Burman design and responses.
| | Std run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Factor 5 | Factor 6 | Factor 7 | Factor 8 | Factor 9 | Factor 10 | Response 1 | Response 2 | | A: substrate | B: moist content | C: inoculum size | D: time | E: K2HPO4 | F: KH2PO4 | G: NaCl | H: MgSO4 | J: CaCl2 | K: NaNO3 | Protease activity | Amylase activity | | % | % | % | h | % | % | % | % | % | % | U/g | U/g |
| | 1 | 10 | 75 | 3 | 72 | 1 | 1 | 0.1 | 0.1 | 0.01 | 1 | 311.87 | 868.76 | | 2 | 5 | 75 | 5 | 24 | 1 | 1 | 0.5 | 0.1 | 0.01 | 0.01 | 74.27 | 354.59 | | 3 | 10 | 50 | 5 | 72 | 0.1 | 1 | 0.5 | 1 | 0.01 | 0.01 | 697.35 | 1775.04 | | 4 | 5 | 75 | 3 | 72 | 1 | 0.1 | 0.5 | 1 | 0.05 | 0.01 | 273.19 | 542.51 | | 5 | 5 | 50 | 5 | 24 | 1 | 1 | 0.1 | 1 | 0.05 | 1 | 79.57 | 638.55 | | 6 | 5 | 50 | 3 | 72 | 0.1 | 1 | 0.5 | 0.1 | 0.05 | 1 | 475.02 | 1485.09 | | 7 | 10 | 50 | 3 | 24 | 1 | 0.1 | 0.5 | 1 | 0.01 | 1 | 75.05 | 471.46 | | 8 | 10 | 75 | 3 | 24 | 0.1 | 1 | 0.1 | 1 | 0.05 | 0.01 | 85.67 | 429.88 | | 9 | 10 | 75 | 5 | 24 | 0.1 | 0.1 | 0.5 | 0.1 | 0.05 | 1 | 120.86 | 486.66 | | 10 | 5 | 75 | 5 | 72 | 0.1 | 0.1 | 0.1 | 1 | 0.01 | 1 | 296.17 | 451.95 | | 11 | 10 | 50 | 5 | 72 | 1 | 0.1 | 0.1 | 0.1 | 0.05 | 0.01 | 530.86 | 1420.33 | | 12 | 5 | 50 | 3 | 24 | 0.1 | 0.1 | 0.1 | 0.1 | 0.01 | 0.01 | 73.71 | 391.6 |
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