Research Article
Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis
Table 4
Cellulase activities and protein concentration for the 24 FFD for medium optimisation.
| | Run | A
a | B
a | C
a | D
a | FPase activity (U·L−1) | Endoglucanase activity (U·L−1) | -Glucosidase activity (U·L−1) | Protein concentration (mg·L−1) |
| | 1 | 4.0 | 0.6 | 0.6 | 0.5 | 123.5 ± 33.1 | 2984.6 ± 39.1 | 284.8 ± 4.7 | 70.9 ± 3.3 | | 2 | 8.0 | 0.6 | 0.6 | 0.5 | 128.5 ± 35.9 | 1749.6 ± 35.4 | 222.2 ± 3.1 | 57.9 ± 2.0 | | 3 | 4.0 | 1.8 | 0.6 | 0.5 | 177.0 ± 23.7 | 2100.6 ± 31.4 | 363.8 ± 2.9 | 93.7 ± 2.4 | | 4 | 8.0 | 1.8 | 0.6 | 0.5 | 89.7 ± 9.3 | 2356.3 ± 10.3 | 243.6 ± 10.3 | 72.1 ± 6.5 | | 5 | 4.0 | 0.6 | 1.8 | 0.5 | 84.4 ± 6.5 | 496.3 ± 7.1 | 47.7 ± 2.9 | 81.7 ± 4.7 | | 6 | 8.0 | 0.6 | 1.8 | 0.5 | 135.2 ± 15.4 | 3985.6 ± 28.9 | 805.0 ± 5.6 | 119.3 ± 3.6 | | 7 | 4.0 | 1.8 | 1.8 | 0.5 | 105.5 ± 14.4 | 2281.1 ± 29.2 | 233.8 ± 12.4 | 63.3 ± 4.8 | | 8 | 8.0 | 1.8 | 1.8 | 0.5 | 68.4 ± 17.1 | 726.9 ± 7.1 | 176.1 ± 7.3 | 35.8 ± 3.1 | | 9 | 4.0 | 0.6 | 0.6 | 1.5 | 250.4 ± 2.1 | 5509.6 ± 33.8 | 915.3 ± 3.8 | 143.3 ± 1.2 | | 10 | 8.0 | 0.6 | 0.6 | 1.5 | 226.6 ± 2.4 | 4943.1 ± 27.4 | 732.6 ± 8.7 | 150.5 ± 9.1 | | 11 | 4.0 | 1.8 | 0.6 | 1.5 | 178.9 ± 3.3 | 2772.4 ± 9.2 | 439.5 ± 2.2 | 101.5 ± 3.2 | | 12 | 8.0 | 1.8 | 0.6 | 1.5 | 140.7 ± 9.8 | 5344.2 ± 10.8 | 816.5 ± 5.7 | 176.4 ± 8.1 | | 13 | 4.0 | 0.6 | 1.8 | 1.5 | 2.5 ± 1.3 | 451.2 ± 4.1 | 13.2 ± 2.9 | 59.9 ± 2.2 | | 14 | 8.0 | 0.6 | 1.8 | 1.5 | 24.3 ± 2.2 | 588.2 ± 11.5 | 139.9 ± 2.8 | 31.3 ± 2.4 | | 15 | 4.0 | 1.8 | 1.8 | 1.5 | 74.0 ± 1.8 | 56.8 ± 2.1 | 34.6 ± 4.9 | 45.6 ± 0.4 | | 16 | 8.0 | 1.8 | 1.8 | 1.5 | 66.6 ± 3.3 | 207.2 ± 15.3 | 60.9 ± 2.9 | 43.2 ± 2.4 | | 17 (CP) | 6.0 | 1.2 | 1.2 | 1.0 | 158.6 ± 4.1 | 4908.0 ± 23.2 | 1345.8 ± 3.5 | 110.9 ± 0.3 | | 18 (CP) | 6.0 | 1.2 | 1.2 | 1.0 | 170.3 ± 2.4 | 5153.7 ± 27.9 | 1320.3 ± 4.1 | 144.8 ± 4.8 | | 19 (CP) | 6.0 | 1.2 | 1.2 | 1.0 | 172.7 ± 1.1 | 5660.0 ± 32.6 | 1326.0 ± 5.9 | 153.5 ± 3.9 | | 20 (CP) | 6.0 | 1.2 | 1.2 | 1.0 | 178.2 ± 1.8 | 4481.9 ± 28.6 | 1234.7 ± 13.9 | 77.6 ± 1.5 | | 21 (CP) | 6.0 | 1.2 | 1.2 | 1.0 | 161.7 ± 7.7 | 5190.5 ± 23.5 | 1128.5 ± 11.2 | 128.1 ± 1.3 |
|
|
A, B, C, D: see Table 1. CP: center point.
|
