Research Article
Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis
Table 5
Cellulase activities and protein concentration for the 32 FFD using an instrumented bioreactor.
| Run | E
a | F
a | FPase activity (U·L−1) | Endoglucanase activity (U·L−1) | -Glucosidase activity (U·L−1) | Protein concentration (mg·L−1) |
| 1 | 100 | 0.2 | 125.6 ± 6.8 | 3184.5 ± 18.2 | 905.9 ± 7.4 | 52.5 ± 0.7 | 2 | 100 | 0.6 | 78.0 ± 3.4 | 2584.9 ± 14.3 | 771.8 ± 6.0 | 47.3 ± 1.8 | 3 | 100 | 1.0 | 163.6 ± 12.8 | 770.5 ± 2.6 | 185.0 ± 3.5 | 91.3 ± 2.9 | 4 | 200 | 0.2 | 190.8 ± 5.3 | 3706.6 ± 17.1 | 984.9 ± 8.1 | 48.8 ± 2.2 | 5 (CP) | 200 | 0.6 | 444.8 ± 8.1 | 9579.5 ± 22.2 | 2395.2 ± 18.3 | 179.2 ± 3.2 | 6 | 200 | 1.0 | 558.6 ± 13.2 | 5622.6 ± 27.4 | 1613.3 ± 19.5 | 209.4 ± 1.0 | 7 | 300 | 0.2 | 252.7 ± 3.7 | 4661.0 ± 15.5 | 1149.0 ± 13.5 | 104.7 ± 2.8 | 8 | 300 | 0.6 | 338.2 ± 4.9 | 9804.4 ± 15.3 | 2860.5 ± 3.5 | 105.5 ± 2.3 | 9 | 300 | 1.0 | 224.4 ± 9.1 | 4535.9 ± 13.8 | 1173.7 ± 6.5 | 125.3 ± 5.5 | 10 (CP) | 200 | 0.6 | 449.5 ± 3.9 | 9784.3 ± 27.4 | 2451.0 ± 15.2 | 187.5 ± 1.9 | 11 (CP) | 200 | 0.6 | 431.2 ± 5.1 | 9396.8 ± 27.1 | 2088.7 ± 13.9 | 176.3 ± 1.5 |
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E, F: see Table 2. CP: center point.
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