Research Article
Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis
Table 6
Experimental validation of optimal conditions for cellulase production, predicted using the multivariate desirability function.
| | | Agitation (rpm) | Aeration (vvm) | FPase activity (U·L−1) | Endoglucanase activity (U·L−1) | -Glucosidase activity (U·L−1) | Protein concentration (mg·L−1) |
| | Predicted values | | FPase activity (U·L−1) | 215 | 0.9 | 460 | — | — | — | | Endoglucanase activity (U·L−1) | 245 | 0.6 | — | 9626 | — | — | | -Glucosidase activity (U·L−1) | 260 | 0.6 | — | — | 2467 | — | | Protein concentration (mg·L−1) | 214 | 1.0 | — | — | — | 189 | | Desirability | 227.5 | 0.68 | 446 | 9414 | 2386 | 174 |
| | Observed values | | Bioreactor (120 h) | 220 | 0.6 | 508 ± 12.1 | 9204 ± 142.2 | 2395 ± 24.8 | 245 ± 15.9 |
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