Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study

Table 1

Data from the temperature dependence of (Figure 1(c)), SCM (Figure 2(c)), and residual catalytic activity (Figure 2(d)) were fitted to (1) to obtain the midpoint of irreversible transitions (apparent ).

	Parameter				Calorimetric transition
			SCM	% activity	Irreversible	Reversible
(°C)		54.3; 66.0			58.71	58.9

Data in Figure 2(b) inset were fitted to (1) in the 19–60°C and 57–72°C. Midpoint transitions were obtained from the calorimetric irreversible process of 1.1 mg·mL⁻¹ protein (apparent , Figure 4(a)) at 30°C·h⁻¹ scan rate and from the reversible transition of 0.56 mg·mL⁻¹ protein (, not shown) at 30°C·h⁻¹ scan rate.