Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study
Table 3
Data from the excess heat capacity versus temperature curves in Figure 5(a) were fitted to (4). The changes in both the calorimetric enthalpy () and the van’t Hoff enthalpy () were obtained through the deconvolution analysis of data ( ).
Transition
1
2
3
4
5
6
Global
(°C)
53.2
55.3
57.4
59.3
60.9
62.9
59.4
(kCal·mol−1)
33.4
79.2
111.2
121.4
120.5
18.8
481.9
(kCal·mol−1)
313.3
250.2
241.9
255.4
257.2
365.3
ND
ND is not determined as the global process is not a two-state process.