Purification and Characterization of Glucose-6-Phosphate Dehydrogenase from Camel Liver

Figure 1

A typical elution profile for the chromatography of the camel liver ammonium sulfate fraction on DEAE-cellulose column (12 cm × 2.4 cm) previously equilibrated with 0.02 M Tris/HCI buffer, pH 7.6 containing 1 mM β-mercaptoethanol, and 1 mM EDTA. The proteins were eluted by a stepwise gradient of NaCl ranging from 0 to 1 M in the equilibration buffer. 5 mL fractions were collected at a flow rate of 60 mL/h.