Research Article
Pseudomonas aeruginosa Exopolyphosphatase Is Also a Polyphosphate: ADP Phosphotransferase
Table 3
Effect of mono- and divalent cations on phosphotransferase activity of the full-length paPpx and of the N-paPpx variant.
| | Mono- or divalent cations | paPpx | N-paPpx | | Phosphotransferase activity | Phosphotransferase activity | | (nmol ATP·min−1·mg−1) | (nmol ATP·min−1·mg−1) |
| | Mg2+ (5 mM) | 193.4 ± 6.6 | 39.3 ± 1.2 | | K+ (80 mM) | 1.2 ± 0.1 | 0.9 ± 0.1 | | Mg2+/K+ (5 mM/80 mM) | 187.6 ± 9.4 | 38.7 ± 2.5 | | (25 mM) | 2.1 ± 0.9 | 1.6 ± 0.4 | | Mg2+/ (5 mM/25 mM) | 189 ± 10.1 | 42.9 ± 2.9 |
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Values are means ± SD of at least three experiments performed independently.
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