Enzyme Research

Enzyme Research / 2015 / Article / Tab 5

Research Article

Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries

Table 5

Proteolytic activity of the papain in the presence of glutaraldehyde, polyethyleneimine, and tripolyphosphate, for 120 minutes at 27°C.

Concentration (%)Glutaraldehyde (U/mL)Polyethyleneimine (PEI) (U/mL)Sodium tripolyphosphate (U/mL)

Control2.04 ± 0.04a2.68 ± 0.09a2.61 ± 0.07a
0.10.18 ± 0.06b3.01 ± 0.04b1.68 ± 0.09b
0.50.13 ± 0.01b2.25 ± 0.24c1.47 ± 0.10c
1.00.12 ± 0.00b0.96 ± 0.07d1.43 ± 0.09c
1.50.12 ± 0.02b0.81 ± 0.08d0.88 ± 0.10d
2.00.11 ± 0.02b0.58 ± 0.06d0.55 ± 0.04e
5.00.01 ± 0.01c0.74 ± 0.14d0.48 ± 0.09e
10.00.00 ± 0.00c0.59 ± 0.27d0.34 ± 0.00e

Obs. different letters indicate that they are statistically different (P < 0.05). Papain concentration was 10 g⋅L−1 (1%—w⋅v−1).

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