Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Table 4

Primers used in Tth pol III holoenzyme PCR reactions.

Template DNA	Primer direction	Sequence (5′-3′)	PCR product (bp)
pET Blue-2	Sense	TAATACGACTCACTATAGGG	200
pET Blue-2	Antisense	GTCGTTTTACAACGTCGTGA
pET Blue-2	Sense	TAATACGACTCACTATAGGG	516
pET Blue-2	Antisense	GCTAACGCAGTCAGGAGTATT
pET Blue-2	Sense	CAATACTCCTGACTGCGTTA	1500
pET Blue-2	Antisense	GAATGAAGCCATACCAAACGA
pET Blue-2	Sense	CAATACTCCTGACTGCGTTA	2000
pET Blue-2	Antisense	CACGCTGTAGGTATCTCAGTT
DNA	Sense	CCGTTCTTCTTCGTCATAA	4650
DNA	Antisense	GATGCCGTTCATGACCTGATAA
DNA	Sense	CCGTTCTTCTTCGTCATAA	7500
DNA	Antisense	GCAGCACAAATGCCACAGGTTCAT
DNA	Sense	CCGTTCTTCTTCGTCATAA	12500
DNA	Antisense	CCATATTCTGTGCAATACCAT
DNA	Sense	CCGTTCTTCTTCGTCATAA	15000
DNA	Antisense	CAGGCAGAGTCTCATGTAACT