Research Article

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Table 2

Lipase production by C. viswanathii in solid-state cultivation with different nitrogen sources.

Nitrogen sources (5% w/w)Lipase activity (U/gds)Specific activity (U/mg prot)

Control31.43 ± 4.9111.64 ± 1.81
Yeast extract119.91 ± 11.6865.50 ± 6.38
Corn steep liquor57.96 ± 5.2049.32 ± 5.35
Whey powder61.00 ± 4.2093.53 ± 6.44
Soybean meal40.65 ± 4.8024.12 ± 4.03
Soy protein84.60 ± 4.4176.22 ± 7.62
Cotton seed protein57.95 ± 2.8366.84 ± 3.72

Cultures were carried out on wheat bran plus barley spent grain (1 : 1, w/w), 50% moisture, and 40% (w/w) poultry fat for 5 days, at 28°C. Control was carried out in the absence of nitrogen source.