Research Article

Highly Active and Stable Large Catalase Isolated from a Hydrocarbon Degrading Aspergillus terreus MTCC 6324

Table 1

Purification table for CAT.

FractionsActivity
(105 U mL−1)
Protein
(mg mL−1)
Specific activity
(105 U mg−1)
Fold
purification
Yield %

crude extract 1.2 ± 0.5 2.9 ± 0.8 0.41 ± 0.10 1.0 100
sup-11.7 ± 0.3 1.8 ± 0.5 0.94 ± 0.20 2.3 88.6
pellet-12.7 ± 0.8 5.4 ± 1.0 0.50 ± 0.09 1.2 10.8
sup-22.3 ± 0.4 1.4 ± 0.3 1.64 ± 0.60 4.0 75.0
pellet-20.3 ± 0.1 7.2 ± 1.2 0.04 ± 0.01 0.1 12.4
50% pellet2.4 ± 0.7 7.2 ± 1.9 0.33 ± 0.10 0.8 10.1
80% pellet3.3 ± 1.2 1.4 ± 0.5 2.36 ± 0.50 5.8 62.5
HIC purified14.7 ± 1.4 0.5 ± 0.1 29.40 ± 1.42 71.7 54.4
SEC purified 39.6 ± 2.0 0.6 ± 0.1 66.00 ± 3.97 160.947.0

sup-1 and pellet-1 are supernatant and pellet of 10,000 g centrifugation.
sup-2 and pellet-2 are supernatant and pellet of 20,000  g centrifugation.
50% and 80% pellet are (NH4)2SO4 precipitated fractions.
HIC purified fraction was eluted at 250 mM (NH4)2SO4 concentration.
Each value represents the mean ± standard error at .