Highly Active and Stable Large Catalase Isolated from a Hydrocarbon Degrading Aspergillus terreus MTCC 6324
Table 1
Purification table for CAT.
Fractions
Activity (105 U mL−1)
Protein (mg mL−1)
Specific activity (105 U mg−1)
Fold purification
Yield %
crude extract
1.2 ± 0.5
2.9 ± 0.8
0.41 ± 0.10
1.0
100
sup-1
1.7 ± 0.3
1.8 ± 0.5
0.94 ± 0.20
2.3
88.6
pellet-1
2.7 ± 0.8
5.4 ± 1.0
0.50 ± 0.09
1.2
10.8
sup-2
2.3 ± 0.4
1.4 ± 0.3
1.64 ± 0.60
4.0
75.0
pellet-2
0.3 ± 0.1
7.2 ± 1.2
0.04 ± 0.01
0.1
12.4
50% pellet
2.4 ± 0.7
7.2 ± 1.9
0.33 ± 0.10
0.8
10.1
80% pellet
3.3 ± 1.2
1.4 ± 0.5
2.36 ± 0.50
5.8
62.5
HIC purified
14.7 ± 1.4
0.5 ± 0.1
29.40 ± 1.42
71.7
54.4
SEC purified
39.6 ± 2.0
0.6 ± 0.1
66.00 ± 3.97
160.9
47.0
sup-1 and pellet-1 are supernatant and pellet of 10,000 g centrifugation. sup-2 and pellet-2 are supernatant and pellet of 20,000 g centrifugation. 50% and 80% pellet are (NH4)2SO4 precipitated fractions. HIC purified fraction was eluted at 250 mM (NH4)2SO4 concentration. Each value represents the mean ± standard error at .