Table of Contents Author Guidelines Submit a Manuscript
Enzyme Research
Volume 2016 (2016), Article ID 7517928, 6 pages
http://dx.doi.org/10.1155/2016/7517928
Research Article

Salivary Myeloperoxidase, Assessed by 3,3′-Diaminobenzidine Colorimetry, Can Differentiate Periodontal Patients from Nonperiodontal Subjects

1Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
2Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand
3Research Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with Oral Health, Khon Kaen University, Khon Kaen 40002, Thailand
4Department of Periodontology, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand
5Dental Department, Fang Hospital, Fang District, Chiangmai 50110, Thailand
6Department of Clinical Chemistry, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
7Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen 40002, Thailand
8Department of Clinical Microbiology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
9Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Received 6 January 2016; Revised 22 March 2016; Accepted 19 April 2016

Academic Editor: Hartmut Kuhn

Copyright © 2016 Supaporn Klangprapan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Periodontal diseases, which result from inflammation of tooth supporting tissues, are highly prevalent worldwide. Myeloperoxidase (MPO), from certain white blood cells in saliva, is a biomarker for inflammation. We report our study on the salivary MPO activity and its association with severity of periodontal diseases among Thai patients. Periodontally healthy subjects () and gingivitis () and periodontitis patients () were enrolled. Assessments of clinically periodontal parameters were reported as percentages for gingival bleeding index (GI) and bleeding on probing (BOP), whereas pocket depth (PD) and clinical attachment loss (CAL) were measured in millimeters and then made to index scores. Salivary MPO activity was measured by colorimetry using 3,3′-diaminobenzidine as substrate. The results showed that salivary MPO activity in periodontitis patients was significantly higher than in healthy subjects () and higher than in gingivitis patients (). No difference was found between gingivitis and healthy groups (). Significant correlations were observed () between salivary MPO activity and GI (, ), BOP (, ), PD (, ), and CAL (, ) index scores. Sensitivity (94.12%), specificity (54.55%), and positive (90.57%) and negative (66.67%) predictive values indicate that salivary MPO activity has potential use as a screening marker for oral health of the Thai community.