Figure 2: Treatment PBED kinetic follow-up. Treatment 1 [PBED-: 500 mL Erlenmeyer flask containing 150 mL media, 10% inoculum (v/v), 1.0 mM CuSO4, 30 gL−1 glucose, 5 mM NH4SO4, 10 gL−1 peptone, and 5 gL−1 yeast extract], enzy. act. 4.69 ± 0.39 UL−1 at 168 h of culture. Assay was carried out in triplicate ().