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Enzyme Research
Volume 2017 (2017), Article ID 5947581, 10 pages
https://doi.org/10.1155/2017/5947581
Research Article

Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

1Laboratorio de Microbiología Ambiental y de Suelos, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana (PUJ), Bogotá, Colombia
2Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Manizales, Caldas, Colombia
3Laboratorio de Biotecnología Molecular, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana (PUJ), Bogotá, Colombia
4Laboratorio de Expresión de Proteínas, Instituto de Errores Innatos del Metabolismo (IEIM), Facultad de Ciencias, Pontificia Universidad Javeriana (PUJ), Bogotá, Colombia
5Laboratorio de Parasitología Molecular, Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana (PUJ), Bogotá, Colombia

Correspondence should be addressed to Raúl A. Poutou-Piñales; oc.ude.anairevaj@uotuopr

Received 11 January 2017; Revised 27 February 2017; Accepted 9 March 2017; Published 21 March 2017

Academic Editor: Hartmut Kuhn

Copyright © 2017 Edwin D. Morales-Álvarez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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