Figure 4: Effect of temperature and pH on reaction of recombinant T. reesei CBHII. The enzyme activity of recombinant T. reesei CBHII produced in W. anomalus 54-A was evaluated at different pH and temperatures reactions condition. Each experiment was carried out in triplicate. Activity is reported as % Relative Activity as compared with the highest activity obtained in the experiment (40°C, pH 5).