“In Silico” Characterization of 3-Phytase A and 3-Phytase B from Aspergillus niger

Table 3

Possible N-glycosylation sites. Three phytases A and B from A. niger.

aa position		Potential	Jury agreement	N-Glyc
Prediction of N-glycosylation sites for 3-phytase A (PDB ID: 3K4Q)
(1)	27 NQSS	0.5302	(6/9)	+
(2)	59 NESV	0.6564	(9/9)	++
(3)	105 NATT	0.6414	(7/9)	+
(4)	120 NYSL	0.7272	(9/9)	++
(5)	207 NNTL	0.5930	(7/9)	+
(6)	230 NFTA	0.6720	(8/9)	+
(7)	339 NHTL	0.4021	(7/9)	−
(8)	352 NSTL	0.7211	(9/9)	++
(9)	376 NGTK	0.7904	(9/9)	+++
(10)	388 NITQ	0.6418	(8/9)	+
Prediction of N-glycosylation sites for Chain A of 3-phytase B (PDB ID: 1QFX)
(1)	87 NTTE	0.4822	(4/9)	−
(2)	172 NYST	0.6708	(8/9)	+
(3)	208 NLTY	0.7573	(9/9)	+++
(4)	231 NLTA	0.6870	(9/9)	++
(5)	296 NASL	0.5727	(6/9)	+
(6)	321 NITP	0.1572	(9/9)	−−−
(7)	406 NYTS	0.6257	(8/9)	+
(8)	423 NVSA	0.5649	(5/9)	+
(9)	439 NTTT	0.5323	(7/9)	+