Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR)

Figure 10

Effect of ionic strength on the apparent lag time of T. crassiceps TGR. Enzyme assays and incubation conditions were as described under Materials and Methods. (a) Representative full progress curves of GSSG reductase activity determined in the presence of 50 μM NADPH and 600 μM GSSG at the following concentrations of NaCl: (●) control without NaCl; (○) 0.2 M NaCl; (▲) 0.4 M NaCl; (Δ) 0.6 M NaCl. To avoid overlapping of traces, curves were displaced vertically. (b) Dependence of the apparent lag time on NaCl concentration. Enzyme assays were carried out in the presence of either 300 μM GSSG (●) or 600 μM GSSG (○). Each point represents mean ± standard deviation (n=6).

(a)

(b)