Figure 1: Purification and activity of AChEs from A. sexdens (AsAChE). Elution profile of ion exchange chromatography (left). Fractions were eluted with a nonlinear gradient of 50 mM phosphate buffer, 1 M NaCl, pH 8.0. AChE activity of free enzyme is shown with dotted line. Zymography on 15% native-PAGE using acetylthiocholine iodide (ATCh) as a substrate (right). Lane 1, crude extract; lane 2, fraction after sulphate ammonium precipitation and applied onto DEAE FF column; lane 3, AsAChE-B and lane 4, AsAChE-A.