Acetylcholinesterases from Leaf-Cutting ant Atta sexdens: Purification, Characterization, and Capillary Reactors for On-Flow Assays

Table 2

Peptides identified in AsAChE-A and AsAChE-B by mass spectrometry.

Sample	Accession number	Identified protein (organism)	Peptide coverage sequences
AsAChE-A	A0A151I5M4 (KYM90380.1)	AChE (Atta colombica)	(1) FAYTGMPTVTETEWPSYTR
			(2) GILQSGTLNAPWSYMTGEKANEVAR
			(3) GILQSGTLNAPWSYmTGEKANEVAR
	A0A158NQX6	Carboxylic ester hydrolase (Atta cephalotes)	(4) YSDFLGDEFFVR
			(5) HYFGNEEIAEKTLK
			(6) HYFGNEEIAEK
			(7) SSNPVFPEHPK
	A0A158NS84	Carboxylic ester hydrolase (Atta cephalotes)	(8) DQFISAVSELNPYVNQIGR
			(9) SVDAWFGIPYAQKPVGPLR
AsAChE-B	EGI67049.1	AChE (Acromyrmex echinatior)	(10) DQFISAVSELNPYVNQIGR
			(11) IVGDYQFTcNVNEFAGR
			(12) YADTGHTVYMYYYK
			(13) HLFNQAIMQSGSATAPWAIISRDESIVR
			(14) GYTHEEIQLSKR
			(15) LAEAVGcPHDR
	EGI59491.1	AChE (Acromyrmex echinatior)	(16) FAYTGMPTVTETEWPSYTR
			(17) FAYTGmPTVTETEWPSYTR
			(18) SLEYTDNERDLSLR
	EGI59490.1	AChE (Acromyrmex echinatior)	(19) TVLDREVHVFYGVPFAKPPVGPLR
			(20) GILQSGTLNAPWSYMTGEK
	A0A151I5M4 (KYM90380.1)	AChE (Atta colombica)	(21) GILQSGTLNAPWSYMTGEKANEVAR
			(22) GILQSGTLNAPWSYmTGEKANEVAR
			(23) TTAcAFWNEFLPR
			(24) YFIWNAEKK