Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
Table 7
Effect of vegetable oils on the lipase production of F. solani F61 using tryptone or ammonium chloride as a nitrogen source.
Carbon sources
Concentration (%)
Biomass (g/L)
Hydrolytic activity (U/mL)
(U/g)
Esterification activity (U/mL)
Tryptone
Castor oil
1
13.99 ± 0.02 b 1
3.77 ± 0.01 e
339.30
0.09 ± 0.01 c
Pequi Oil
1
13.6 ± 0.02 d
2.88 ± 0.01 g
273.60
0.10 ± 0.00 b
Babassu Oil
1
14.5 ± 0.05 a
3.50 ± 0.01 f
192.50
0.03 ± 0.00 f
Copaiba Oil
1
8.98 ± 0.03 g
0.29 ± 0.01 h
29.00
0.07 ± 0.01 d
Buriti oil
1
13.14 ± 0.02 e
4.51 ± 0.01 d
451.00
0.14 ± 0.00 a
Degummed Soybean Oil
1
13.95 ± 0.03 c
6.17 ± 0.01 b
524.45
0.07 ± 0.00 d
Cotton Oil Degomado
1
13.94 ± 0.02 c
8.14 ± 0.01 a
732.60
0.05 ± 0.00 e
Frying Oil
1
13.00 ± 0.04 f
5.02 ± 0.01 c
351.40
0.05 ± 0.00 e
Ammonium chloride
Castor oil
1
8.64 ± 0.02 a
0.18 ± 0.01 d
19.80
0.40 ± 0.03 c
Pequi Oil
1
5.70 ± 0.04 c
0.12 ± 0.01 f
14.40
0.30 ± 0.02 e
Babassu Oil
1
4.59 ± 0.02 e
0.88 ± 0.01 a
105.60
0.41 ± 0.02 b
Copaiba Oil
1
3.03 ± 0.01 g
0.12 ± 0.01 f
13.80
0.43 ± 0.01 a
Buriti oil
1
5.98 ± 0.01 b
0.24 ± 0.01 c
27.60
0.36 ± 0.01 d
Degummed Soybean Oil
1
5.10 ± 0.01 d
0.14 ± 0.01 e
14.00
0.36 ± 0.01 d
Cotton Oil Degomado
1
4.02 ± 0.01 f
0.39 ± 0.01 b
42.90
0.28 ± 0.02 g
Frying Oil
1
1.74 ± 0.01 h
0.14 ± 0.01 e
16.10
0.29 ± 0.01 f
Culture conditions: submerged cultivation was carried out in medium containing g/L- KH2PO4 – 1, MgSO4.H2O – 1.123 e CuSO4 – 0.06. Supplemented with 2.0% Tryptone and 2.0% Ammonium chloride at 28°C, 180 rpm for 5 days. Label: n.d. no activity detected under the assay conditions. 1averages followed by distinct letters differ by Scott-Knott test at the level of significance 0.05. Pearson correlation was determined using biomass and hydrolytic activity. R-Pearson correlation: 0.67 for Tryptone 2.0% and R-Pearson correlation: -0.03 for Ammonium chloride 2.0%.
