Characterization of the Gene Expression Profile of Human Hippocampus in Mesial Temporal Lobe Epilepsy with Hippocampal Sclerosis

Table 3

Comparison of the averaged values of individual epileptic patients and controls between microarray and RT-PCR analysis for genes, which are suspected to be involved in medically intractable epilepsy.

		Gene array^1		RT-PCR^2[Ct values]^3
Genes	Symbol	Control	AHE	Control	AHE
ATP-binding cassette, sub-family B (MDR1/TAP), member 1	ABCB1/P-gp	136.3 ± 26.2	164.3 ± 60.5 (𝑃=.469)*	1.2 ± 0.1 [30.1 ± 0.6]	2.3 ± 0.5 (𝑃=.030) [28.3 ± 0.6]
Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)	ABCB2/TAP1	58.7 ± 12.3	97.8 ± 15.2 (𝑃=.251)	0.9 ± 0.1 [31.9 ± 0.5]	2.1 ± 0.8 (𝑃=.020) [29.7 ± 0.8]
Transporter 2, ATP-binding cassette, subfamily B (MDR/TAP)	ABCB3/TAP2	42.3 ± 9.6	67.9 ± 12.5 (𝑃=.163)	1.1 ± 0.2 [32.8 ± 0.9]	2.1 ± 0.8 (𝑃=.023) [30.1 ± 1.0]
ATP-binding cassette, sub-family C (CFTR/MRP), member 4	ABCC4/MRP4	104.7 ± 7.2	235.0 ± 69.4 (𝑃=.012)	1.2 ± 0.2 [31.1 ± 0.5]	6.4 ± 2.1 (𝑃=.030) [28.7 ± 0.7]

¹The microarray data are presented as raw values calculated by Microarray Suite 5. The data are given as means ± SD of three controls and eight epileptic patients for each gene, respectively.
²The results of RT-PCR analysis are expressed as relative gene expression and normalized with 18S rRNA expression as reference gene.
Data are presented as mean ± SD of three controls. *The statistical differences between AHE and controls are indicated (𝑃-value, Student’s t-Test).
³Threshold cycles [CT-values] are given as mean ± SD