The Epigenetic Repertoire of Daphnia magna Includes Modified Histones

Figure 1

Immunohybridization of Daphnia magna embryos with antibodies specific to modified histone H3 and H4. Immunohybridization of protein extracted from Daphnia embryos with antibodies to histone H3 trimethyl K27 (H3K27me3), histone H4 dimethyl K20 (H4K20me2), histone H3 acetyl K14 (H3K14ac), histone H3 dimethyl K4 (H3K4me2), and histone H3 monomethyl K9 (H3K9me). These antibodies all detect a strong band at 17 kDa, the expected size for histone H3 and histone H4.